Active standard | Published: 01/12/2022

ČSN P CEN/TS 17781

Organic, organo-mineral and inorganic fertilizers - Detection of Escherichia coli

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Detail information

Designation: ČSN P CEN/TS 17781

Publication date: 01/12/2022

Pages: 24

Country: Czech technical standard

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Anotation

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009. However, the present method was not validated for blends.
This document specifies a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for the detection of the enzyme ß-glucuronidase. The method is based on ISO 16649-2.
Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected. Detected microorganisms are presumptively determined ß-glucuronidase-positive Escherichia coli, since some Enterobacteriaceae, in particular Shigella and Salmonella, can also show ß-glucuronidase activity at 44 °C
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