Active standard | Published: 18/07/2016

ISO/TS 19006:2016

Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
(Nanotechnologies — Essai au diacétate de 5-(et 6)- Chlorométhyle -2’,7’ Dichloro-dihydro-fluorescéine (CM-H2DCF-DA) pour l’évaluation de la génération intracellulaire d’especes réactives a l´oxygene induites par les nanoparticules sur la lignée souche 264.7 de macrophages)

Available languages: English

Available design: electronic design (pdf), Print design

from 199.60 USD show on eshop

Detail information

Designation: ISO/TS 19006:2016

Publication date: 18/07/2016

Pages: 21

Country: International technical standard

Where to buy?

You can buy at www.mystandards.biz

Anotation

Description / Abstract: ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay. The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

Popular Publishers

full catalog