Aktívna norma | Vydaná: 18.07.2016

ISO/TS 19006:2016

Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
(Nanotechnologies — Essai au diacétate de 5-(et 6)- Chlorométhyle -2’,7’ Dichloro-dihydro-fluorescéine (CM-H2DCF-DA) pour l’évaluation de la génération intracellulaire d’especes réactives a l´oxygene induites par les nanoparticules sur la lignée souche 264.7 de macrophages)

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Podrobné informácie

Označenie: ISO/TS 19006:2016

Dátum vydania: 18.07.2016

Stránok: 21

Krajina: Medzinárodná technická norma

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Anotácia

Description / Abstract: ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay. The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

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